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Image Search Results
Journal:
Article Title: Enhanced microtubule-dependent trafficking and p53 nuclear accumulation by suppression of microtubule dynamics
doi: 10.1073/pnas.132275599
Figure Lengend Snippet: Treatment with low concentrations of PTX or VCR followed by ADR enhances apoptosis in A549 cells. (A) A549 cells were treated with 3, 6, or 100 nM of PTX or VCR for 18 hr followed by treatment with 200 ng/ml of ADR for an additional 6 hr (PTX3→ADR, PTX6→ADR, PTX100→ADR, and VCR3→ADR, VCR6→ADR, VCR100→ADR). ADR was added to the medium containing PTX or VCR at the indicated concentrations. Untreated cells (CTRL) or cells treated with 200 ng/ml of ADR alone (ADR), PTX alone, or VCR alone for 24 hr (PTX3, PTX6, PTX100; VCR3, VCR6, VCR100) were included as controls. Western blots were probed with antibodies against the cleaved (p85) form of PARP, p53, mdm2, and actin as a loading control. (B) A549 cells were treated with 3, 6, or 100 nM PTX for 18 hr, followed by treatment with 200 ng/ml of ADR for an additional 6 hr (PTX3→ADR, PTX6→ADR, PTX100→ADR). Untreated cells (CTRL) or cells treated with PTX alone for 24 hr (PTX3, PTX6, PTX100) are included as controls. Ten micrograms of total RNA from each sample were analyzed by Northern blotting. Ethidium bromide staining of 28S RNA is shown as loading control.
Article Snippet: The antibody against the cleaved epitope of poly(
Techniques: Western Blot, Northern Blot, Staining